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A fully automated FAIMS-DIA mass spectrometry-based proteomic pipeline

Luke Reilly1, Erika Lara1, Daniel Ramos1

  • 1Center for Alzheimer's and Related Dementias (CARD), National Institute on Aging and National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.

Cell Reports Methods
|September 20, 2023
PubMed
Summary

This study introduces a streamlined proteomics pipeline for deep cellular proteome coverage. The automated workflow, using FAIMS-DIA and Spectronaut, identifies over 9,000 proteins in iPSC-derived neurons with high accuracy and reproducibility.

Keywords:
CP: BiotechnologyCP: Systems biologyDDADIAFAIMSautomationdatabase searchmass spectrometryproteomicssingle-shot MS

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