Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prospective Cohort Study Evaluating Impact of Hemodialysis on Glycemia: BLOSSOM (Blood Sugar Sensing on Maintenance Dialysis) Cohort Findings.

Clinical journal of the American Society of Nephrology : CJASN·2026

Same author

Associations of maternal urinary perchlorate, thiocyanate, and nitrate with maternal thyroid function during pregnancy: Modification by iodine status in the MARBLES study.

Environmental research·2026

Same author

Development and Implementation of an AI System for Generating Clinical Urine Drug Test Sign-Outs.

JAMA network open·2026

Same author

Interlaboratory comparison with a reference measurement procedure (RMP) for determining 24,25-dihydroxyvitamin D<sub>3</sub> in human serum using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Analytical and bioanalytical chemistry·2026

Same author

Association of the vitamin D metabolite ratio with bone turnover markers and changes in volumetric BMD.

JBMR plus·2026

Same author

Transferability and Between Laboratory Imprecision of an LC-MS/MS-Based Reference Measurement Procedure for Apolipoproteins.

Clinical chemistry·2026

Same journal

Platelet proteome links metabolism to reactivity in Essential Thrombocythemia.

Molecular & cellular proteomics : MCP·2026

Same journal

Genetic rescue of disrupted synaptic protein interaction network dynamics following SYNGAP1 reactivation.

Molecular & cellular proteomics : MCP·2026

Same journal

ASAP-ID: Proximity labelling with small tags.

Molecular & cellular proteomics : MCP·2026

Same journal

Proteome profiling reveals NQO2 activity contributing to proteasome inhibitor resistance in multiple myeloma cell lines.

Molecular & cellular proteomics : MCP·2026

Same journal

Depletion-Free Automated Enrichment of Serum Glycopeptides for High-Throughput Clinical Glycoproteomics.

Molecular & cellular proteomics : MCP·2026

Same journal

Extracellular Vesicles from Glioblastoma Cells Reflect 2D vs. 3D Culture Adaptation and Resistance to Temozolomide.

Molecular & cellular proteomics : MCP·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 16, 2025

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Clinical Proteomics for Solid Organ Tissues.

William S Phipps¹, Mark R Kilgore¹, Jacob J Kennedy²

¹Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA.

Molecular & Cellular Proteomics : MCP

|September 20, 2023

Summary

This summary is machine-generated.

Mass spectrometry offers improved standardization and reproducibility for immunohistochemistry, a critical diagnostic technique. This advanced method enables more accurate protein quantification and multiplexed analysis for better cancer treatment and diagnostics.

Keywords:

clinical proteomics immunohistochemistry mass spectrometry pathology standardization targeted proteomics tissue analysis untargeted proteomics

More Related Videos

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Related Experiment Videos

Last Updated: Jul 16, 2025

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Biomedical diagnostics
Analytical chemistry
Pathology

Background:

Microscopic examination of biopsied solid organ tissue is standard practice.
Immunohistochemistry (IHC) is vital for identifying cell markers and therapeutic targets in diagnostics.
Traditional IHC faces challenges in standardization and reproducibility due to antibody reliance and limited multiplexing.

Purpose of the Study:

To survey challenges in traditional immunohistochemistry.
To explore the potential of mass spectrometry (MS) to address these limitations.
To discuss the clinical utility and standardization of MS in pathology.

Main Methods:

Review of traditional immunohistochemistry methods and their limitations.
Discussion of mass spectrometry techniques, including liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Exploration of targeted MS assays for protein quantification and untargeted MS for multiplexed protein detection.

Main Results:

Targeted MS assays offer robust quantification and improved interlaboratory reproducibility using internal standards.
Untargeted MS enables inherent multiplexing for detecting and quantifying hundreds to thousands of proteins.
Data-independent acquisition MS presents potential strengths for clinical applications, though not yet widely used.

Conclusions:

Mass spectrometry, particularly LC-MS/MS, presents a promising alternative to overcome standardization and reproducibility issues in IHC.
MS facilitates more accurate protein measurements and high-plex analysis, enhancing diagnostic pathology and cancer treatment.
Guidance is necessary for broader clinical adoption and standardization of MS-based assays in pathology.