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Updated: Jul 16, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
An entropy-controlled objective chip for reflective confocal microscopy with subdiffraction-limit resolution
Jun He1, Dong Zhao1, Hong Liu2
1Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, China.
Abstract:
Planar diffractive lenses (PDLs) with optimized but disordered structures can focus light beyond the diffraction limit. However, these disordered structures have inevitably destroyed wide-field imaging capability, limiting their applications in microscopy. Here, we introduce information entropy S to evaluate the disorder of an objective chip by using the probability of its structural deviation from standard Fresnel zone plates. Inspired by the theory of entropy change, we predict an equilibrium point [Formula: see text] to balance wide-field imaging (theoretically evaluated by the Strehl ratio) and subdiffraction-limit focusing. To verify this, a [Formula: see text] objective chip with a record-long focal length of 1 mm is designed with [Formula: see text], which is the nearest to the equilibrium point among all reported PDLs. Consequently, our fabricated chip can focus light with subdiffraction-limit size of 0.44 λ and image fine details with spatial frequencies up to 4000 lp/mm experimentally. These unprecedented performances enable ultracompact reflective confocal microscopy for superresolution imaging.
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