An improved method for the preparation and culture of urothelial cells

Urothelial cells have been prepared by a new method involving collagenase treatment of the lumen of a ureter. These cells have been identified as epithelial and successfully subcultured. In addition, we have observed that growth rate is significantly increased by the inclusion of an extract of bovine hypothalamus in the growth medium. This system for cell preparation and culture should greatly facilitate studies involving urothelial cells.