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Related Concept Videos

Carbon-13 (¹³C) NMR: Overview01:10

Carbon-13 (¹³C) NMR: Overview

5.8K
Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
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First worldwide multicenter validation of the POLARIS preclinical polarizer across biological models and imaging paradigms.

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Related Experiment Video

Updated: Jul 16, 2025

Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
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Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy

Published on: April 21, 2023

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Current Methods for Hyperpolarized [1-13C]pyruvate MRI Human Studies.

Peder Ez Larson1,2, Jenna Ml Bernard1, James A Bankson3

  • 1Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA 94143, USA.

Arxiv
|September 21, 2023
PubMed
Summary
This summary is machine-generated.

Hyperpolarized 13C MRI uses specialized agents to measure metabolism in diseases like cancer. This guide details best practices for human studies using 13C-pyruvate, a key metabolic tracer.

Keywords:
Hyperpolarized MRIcarbon-13dissolution dynamic nuclear polarizationmetabolic imagingpyruvate

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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Last Updated: Jul 16, 2025

Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
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Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

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Area of Science:

  • Medical Imaging
  • Metabolic Imaging
  • Biophysics

Background:

  • Hyperpolarized (HP) 13C MRI measures localized metabolism altered in diseases like cancer, liver, heart, and kidney conditions.
  • Human studies using HP 13C MRI have grown rapidly in the last decade due to improved agent preparation methods.
  • This technique offers insights into in vivo metabolic processes crucial for disease diagnosis and monitoring.

Approach:

  • This paper synthesizes current successful practices for human HP 13C MRI studies, focusing on [1-13C]pyruvate.
  • It covers four critical areas: agent preparation, MRI system setup/calibration, data acquisition/reconstruction, and data analysis/quantification.
  • The consensus document summarizes published studies, current practices, and identifies evidence gaps, strengths, and limitations.

Key Points:

  • [1-13C]pyruvate is the most common HP agent, situated at a key junction in glycolysis.
  • Standardized protocols for preparation, system calibration, acquisition, and analysis are essential for reliable results.
  • The paper provides a comprehensive reference for advancing human metabolic imaging research.

Conclusions:

  • HP 13C MRI, particularly with [1-13C]pyruvate, is a powerful tool for metabolic imaging in human diseases.
  • Establishing consensus on best practices is crucial for the continued advancement and clinical translation of this technology.
  • This work serves as a foundational reference for future research and consensus-building in the field of hyperpolarized MRI.