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A Nanopore sequencing-based pharmacogenomic panel to personalize tuberculosis drug dosing
Renu Verma1,2,3, Kesia Esther da Silva1, Neesha Rockwood4,5,6
1Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, California, USA.
Medrxiv : the Preprint Server for Health Sciences
|September 21, 2023
Summary
A new Nanopore sequencing panel accurately identifies genetic variations affecting tuberculosis drug metabolism. This enables personalized dosing strategies to improve treatment outcomes and reduce adverse drug reactions in patients with active TB.
Area of Science:
- Pharmacogenomics
- Molecular Diagnostics
- Infectious Diseases
Background:
- Standardized anti-tubercular (TB) drug dosing results in variable plasma drug levels, leading to adverse reactions, delayed response, and relapse.
- Genetic mutations significantly contribute to interindividual pharmacokinetic variability in TB drug metabolism.
- A lack of pharmacogenomic (PGx) assays hinders personalized anti-TB drug dosing.
Conclusions:
- Targeted sequencing using a low-cost, portable Nanopore instrument can effectively detect polymorphisms influencing TB drug metabolism.
- This approach facilitates personalized dosing for TB treatment and prevention.
- The developed PGx panel holds promise for improving TB treatment efficacy and safety.
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