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Updated: Jul 16, 2025
Chronic Salmonella Infection Induced Intestinal Fibrosis
Published on: September 22, 2019
Emerging Aeromonas enteric infections: their association with inflammatory bowel disease and novel pathogenic
Seul A Lee1, Fang Liu1, Christopher Yuwono1
1School of Biotechnology and Biomolecular Sciences, University of New South Wales , Sydney, New South Wales, Australia.
Importance:
This study for the first time reports the association between inflammatory bowel disease (IBD) and Aeromonas enteric infection detected by bacterial pathogen cultivation, highlighting the need of clinical and public health attention. The finding that patients with IBD are more susceptible to Aeromonas enteric infection suggests that detection of Aeromonas enteric infection should be routinely performed for the diagnosis and treatment of IBD. This study also reports novel bacterial pathogenic mechanisms employed by Aeromonas veronii. Through comparative transcriptomic analysis and other techniques, this study revealed the pathogenic mechanisms by which A. veronii causes damage to intestinal epithelial cells. Among the various pathogenic mechanisms identified, the downregulating tRNA, small nuclear and nucleolar RNAs in human intestinal epithelial cells are novel bacterial pathogenic mechanisms.
Insights
This study found a significant association between Aeromonas infection and inflammatory bowel disease (IBD). Aeromonas veronii damages intestinal cells through novel mechanisms, including downregulating RNA, warranting clinical attention.
Area of Science:
- Microbiology
- Gastroenterology
- Pathogenomics
Background:
- * Aeromonas species are increasingly recognized as significant enteric pathogens.
- * Inflammatory Bowel Disease (IBD) is a chronic gastrointestinal condition with complex etiology.
- * Understanding the role of enteric pathogens in IBD pathogenesis is crucial for effective management.
Purpose of the Study:
- * To investigate the prevalence of Aeromonas and other enteric pathogens in IBD patients compared to non-IBD individuals.
- * To elucidate the pathogenic mechanisms of Aeromonas veronii in the intestinal epithelium.
- * To identify novel bacterial virulence factors contributing to intestinal damage.
Main Methods:
- * Comparative analysis of enteric bacterial pathogen isolation rates from 2,279 IBD patients and 373,276 non-IBD patients.
- * In vitro study using HT-29 cells as a human intestinal epithelial model to investigate Aeromonas veronii pathogenesis.
- * Transcriptomic analysis to identify host gene expression changes following infection with A. veronii.
- * Investigation of apoptosis, microvilli integrity, and cytokine production (IL-8) in infected epithelial cells.
Main Results:
- * Significantly higher isolation rates of Aeromonas species in IBD patients (OR=2.11, P=0.0001).
- * A. veronii upregulated 177 inflammatory genes and uniquely downregulated 52 protein-coding genes, including tRNAs, snRNAs, and snoRNAs in HT-29 cells.
- * A. veronii induced intestinal epithelial apoptosis via the intrinsic pathway, caused microvilli damage, and stimulated IL-8 production.
Conclusions:
- * This study provides the first evidence linking Aeromonas enteric infection to IBD, detected via bacterial cultivation.
- * A. veronii employs multiple pathogenic mechanisms to damage intestinal epithelial cells, including novel RNA downregulation.
- * Findings suggest routine screening for Aeromonas in IBD patients and highlight A. veronii as a potential contributor to IBD pathogenesis.
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