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Related Experiment Video

Updated: Jun 28, 2026

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Genetic engineering strategy for generating a stable dsRNA virus vector using a virus-like codon-modified transgene.

Yuta Kanai1, Misa Onishi1, Yukino Yoshida1

  • 1Department of Virology, Research Institute for Microbial Diseases, Osaka University , Osaka, Japan.

Journal of Virology
|September 21, 2023
PubMed
Summary
This summary is machine-generated.

Altering transgene codon usage to match the viral genome enhances genetic stability in RNA virus vectors. This virus-like codon modification strategy improves rotavirus and orthoreovirus vector development for gene delivery.

Keywords:
codondsRNA virusmammalian reovirusrotavirusvirus vector

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Area of Science:

  • Molecular biology
  • Virology
  • Genetic engineering

Background:

  • Transgene stability in RNA virus vectors varies, with underlying mechanisms poorly understood.
  • Genetic instability of transgenes limits the efficacy of RNA virus-based gene delivery systems.

Purpose of the Study:

  • To investigate the molecular factors influencing transgene stability in double-stranded RNA virus vectors.
  • To develop a strategy for enhancing transgene stability using codon usage modification.

Main Methods:

  • Analyzed the impact of nucleotide composition on transgene stability.
  • Modified transgene codon usage to mimic that of the viral genome.
  • Generated and assessed the stability of rotavirus and mammalian orthoreovirus vectors with modified transgenes.

Main Results:

  • Transgene stability was significantly affected by nucleotide composition.
  • Altering codon usage to resemble the viral genome substantially increased transgene stability.
  • Successfully generated stable rotavirus and mammalian orthoreovirus vectors using this strategy.

Conclusions:

  • Virus-like codon modification is an effective strategy to enhance transgene stability in double-stranded RNA virus vectors.
  • This approach facilitates the development of robust rotavirus and orthoreovirus vectors for gene delivery.
  • The technology holds potential for broader application across various RNA viruses and delivery targets.