Related Experiment Video
Updated: Jul 16, 2025
10:57
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
13.0K
Bimodal persistent luminescence for autofluorescence-free ratiometric biosensing
Wenjing Dai1, Bing Qi1, Zhihao Li2
1The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123, China.
Analytical and Bioanalytical Chemistry
|September 21, 2023
Summary
This study introduces dual-emissive persistent luminescence nanoparticles (PLNPs) for self-calibrated optical biosensing. The novel strategy eliminates interference, improving accuracy in detecting analytes like urinary mesna in complex samples.
Area of Science:
- Optical biosensing
- Nanoparticle-based diagnostics
- Luminescence spectroscopy
Background:
- Optical biosensing faces challenges from autofluorescence and excitation source fluctuations, reducing sensitivity and accuracy.
- Developing self-calibrating biosensing platforms is crucial for reliable detection in complex matrices.
- Persistent luminescence nanoparticles (PLNPs) offer potential for sensitive detection but require strategies to mitigate interference.
Purpose of the Study:
- To develop a bimodal persistent luminescence strategy for dual-emissive PLNPs with built-in self-calibration.
- To design ZnGa2O4:Cr PLNPs with tunable emission ratios for ratiometric detection.
- To demonstrate the application of these PLNPs for the reliable detection of urinary mesna, eliminating autofluorescence interference.
Main Methods:
- Synthesis of dual-emissive ZnGa2O4:Cr persistent luminescence nanoparticles (PLNPs) with emissions at 490 nm and 695 nm.
- Adjustment of the I490/I695 emission intensity ratio by controlling Cr3+ doping concentration.
- Application of the ratiometric PLNPs for the detection of urinary mesna in biological samples.
Main Results:
- ZnGa2O4:Cr PLNPs exhibited tunable emission ratios, enabling ratiometric detection.
- The ratiometric detection of urinary mesna showed a linear relationship (0-40 μM) with a limit of detection of 0.40 μM.
- Autofluorescence interference from urine was eliminated, and instrumental fluctuations were suppressed, with high recovery rates (99.1–109.0%) for mesna detection.
Conclusions:
- The bimodal persistent luminescence strategy provides a robust self-calibration mechanism for optical biosensing.
- Dual-emissive ZnGa2O4:Cr PLNPs offer a reliable platform for detecting urinary mesna in complex matrices.
- This approach holds promise for advancing optical biosensing applications in disease diagnosis, food safety, and environmental monitoring.
