Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Adrenergic Agonists: Indirect-Acting Agents01:25

Adrenergic Agonists: Indirect-Acting Agents

1.7K
Indirect-acting adrenergic agonists potentiate the effects of endogenous catecholamines through different mechanisms without directly binding to adrenoceptors.
One mechanism involves depleting stored catecholamines by displacing them from synaptic vesicles. These agents, known as "displacers," are transported into vesicles at the expense of noradrenaline. Examples include amphetamine and tyramine, which lack a catechol moiety, resulting in prolonged action, improved oral...
1.7K
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

1.1K
Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
1.1K
Adrenergic Agonists: Direct-Acting Agents01:30

Adrenergic Agonists: Direct-Acting Agents

1.6K
Drugs that mimic the action of endogenous catecholamines like noradrenaline and adrenaline are called adrenergic agonists or sympathomimetics. Based on their mechanism of action, sympathomimetics can be classified as direct-, indirect-, or mixed-acting sympathomimetics. Direct-acting adrenergic agonists activate adrenoceptors without affecting presynaptic neurons, making them independent of neuronal catecholamine-depleting agents like reserpine and guanethidine.
These agents can be classified...
1.6K
Adrenergic Neurons: Neurotransmission01:27

Adrenergic Neurons: Neurotransmission

3.9K
Postganglionic sympathetic fibers (except those supplying the sweat glands) releasing noradrenaline or norepinephrine are called noradrenergic or adrenergic neurons. Noradrenaline, dopamine, adrenaline, or epinephrine are collectively called "catecholamines" as they contain a catechol moiety and an amine side chain. The five stages of neurotransmitter release involve their synthesis, storage, release, reuptake and metabolism.
Synthesis: Catecholamine synthesis requires tyrosine, which...
3.9K
Adrenergic Agonists: Mixed-Action Agents01:28

Adrenergic Agonists: Mixed-Action Agents

771
Mixed-action adrenergic agonists, like ephedrine and pseudoephedrine, directly and indirectly affect adrenergic receptors. These agents stimulate adrenoceptors and indirectly release stored neurotransmitters, amplifying the adrenergic response.
Ephedrine and pseudoephedrine lack a catecholamine group, making them less susceptible to degradation by metabolic enzymes. They have increased oral bioavailability and lipophilicity, resulting in a longer duration of action. Their response is reduced by...
771
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

611
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
611

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Online Electroextraction-NanoESI-MS for Rapid Analysis of Catecholamines in Urine.

Analytical chemistry·2025
Same author

Mass Spectrometry Imaging: Unveiling New Horizons in Antibiotic Research.

Critical reviews in analytical chemistry·2025
Same author

Particle size-dependent neurotoxicity of microplastics in zebrafish (Danio rerio): Spatially resolved lipidomics links metabolic dysregulation to neurological disorders.

Journal of hazardous materials·2025
Same author

Mechanistic Study of Cyclohexane Oxidation to KA Oil Enabled by Circulating Microdroplets.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Air-liquid interfaces of the water microdroplets as nanoreactors: Environmental modulation of catecholamine oxidation and oxidative damage.

The Journal of chemical physics·2025
Same author

Proteomic analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 responding to hyper- and hyposalinity stress.

World journal of microbiology & biotechnology·2025

Related Experiment Video

Updated: Jul 16, 2025

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
13:35

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

14.7K

Recent Advances in Catecholamines Analytical Detection Methods and Their Pretreatment Technologies.

Jie Jiang1,2,3, Meng Zhang1,2, Zhilong Xu1,2

  • 1School of Marine Science and Technology, Harbin Institute of Technology (WeiHai), Weihai, Shandong, China.

Critical Reviews in Analytical Chemistry
|September 21, 2023
PubMed
Summary

This review summarizes analytical detection technologies for catecholamines (CAs), vital hormones and neurotransmitters. It covers advancements in pretreatment and detection methods for accurate CA measurement, aiding disease diagnosis.

Keywords:
Catecholaminesanalytical methodsbiological sampleneurotransmitterpretreatment technology

More Related Videos

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
08:41

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

9.4K
Author Spotlight: An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry
04:19

Author Spotlight: An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry

Published on: March 10, 2023

1.8K

Related Experiment Videos

Last Updated: Jul 16, 2025

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
13:35

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

14.7K
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
08:41

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

9.4K
Author Spotlight: An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry
04:19

Author Spotlight: An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry

Published on: March 10, 2023

1.8K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Neuroscience

Background:

  • Catecholamines (CAs), including adrenaline, noradrenaline, and dopamine, are crucial neurotransmitters and hormones regulating key physiological processes.
  • Accurate measurement of CAs is vital for understanding cardiovascular, metabolic, and stress-related functions.
  • Existing analytical methods for CAs have limitations, necessitating comprehensive reviews of recent advancements.

Purpose of the Study:

  • To provide a comprehensive summary of recent analytical detection technology research on CAs (2017-2022).
  • To discuss the advantages and limitations of various CA detection and pretreatment methods.
  • To offer perspectives on developing novel methods for CA analysis in disease diagnosis and research.

Main Methods:

  • Literature review focusing on analytical detection technologies for CAs published between 2017 and 2022.
  • Analysis of various detection methods: LC-MS, fluorescence, colorimetric, SERS, SPR.
  • Evaluation of efficient pretreatment technologies for CAs in biological matrices.

Main Results:

  • Significant progress in LC-MS detectors for real-time CA monitoring.
  • Development of diverse detection methods with varying degrees of success.
  • Flourishing of efficient pretreatment technologies with selective and recoverable materials.

Conclusions:

  • A comprehensive summary of CA analytical detection technology is currently lacking.
  • This review highlights the importance of accurate CA measurement for disease diagnosis and research.
  • Perspectives are offered for the development of novel CA detection and pretreatment methods.