Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices

Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection. We then detail realization of slices and patch-clamp recordings. While we use, as an example, tanycytes of the arcuate nucleus of the hypothalamus and pro-opiomelanocortin neurons, this protocol can be adapted to study metabolic coupling between tanycytes and any neuronal population. For complete details on the use and execution of this protocol, please refer to Lhomme et al. (2021).¹.