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Related Experiment Video

Updated: Jul 16, 2025

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

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Published on: November 17, 2017

Phenotypic analysis with trans-recombination-based genetic mosaic models.

Yu Zhang¹, Jianhao Zeng², Bing Xu¹

¹School of Life Sciences, Nantong University, Nantong, Jiangsu, China.

The Journal of Biological Chemistry

|September 21, 2023

Summary

This summary is machine-generated.

Mosaicism, the presence of distinct cell populations, is studied using advanced genetic models. These models enable precise lineage tracing and cell-autonomous gene function analysis in development and disease research.

Keywords:

Drosophila MADM MARCM cancer cell–cell interaction genomic imprinting lineage tracing mouse zebrafish

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Area of Science:

Developmental Biology
Genetics
Cell Biology

Background:

Mosaicism involves genetically distinct cell populations arising from a single zygote.
It occurs during development, aging, and in genetic diseases.
Genetically engineered mosaic analysis models offer high cellular and spatiotemporal resolution for gene function studies.

Purpose of the Study:

To provide a comprehensive review of established mosaic analysis models.
To highlight the applications of these models in biological research.
To emphasize their utility in understanding development and disease mechanisms.

Main Methods:

Review of genetic mosaic analysis models, specifically Mosaic Analysis with a Repressible Cellular Marker (MARCM) and Mosaic Analysis with Double Markers (MADM).
These models utilize trans-recombination to generate sibling cells with distinct genotypes within the same organism.
Simultaneous labeling of these sibling cells with different colors facilitates lineage tracing.

Main Results:

MARCM and MADM systems enable the generation of genetically distinct sibling cells within a single animal.
These systems allow for simultaneous multicolor labeling for lineage tracing.
They are powerful tools for studying cell-autonomous versus non-autonomous gene functions.

Conclusions:

Mosaic analysis models are crucial for detailed lineage tracing and gene function studies.
These models facilitate the determination of cell-autonomous and non-autonomous gene functions.
Leveraging these models will advance discoveries in the cellular and molecular mechanisms of development and disease.