Related Experiment Video
Updated: Jul 16, 2025

MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
Published on: February 22, 2017
Exploring the host range for genetic transfer of magnetic organelle biosynthesis
Marina V Dziuba1, Frank-Dietrich Müller1, Mihály Pósfai2,3
1Department of Microbiology, Faculty of Biology, Chemistry and Geosciences, University of Bayreuth, Bayreuth, Germany.
Abstract:
Magnetosomes produced by magnetotactic bacteria have great potential for application in biotechnology and medicine due to their unique physicochemical properties and high biocompatibility. Attempts to transfer the genes for magnetosome biosynthesis into non-magnetic organisms have had mixed results. Here we report on a systematic study to identify key components needed for magnetosome biosynthesis after gene transfer. We transfer magnetosome genes to 25 proteobacterial hosts, generating seven new magnetosome-producing strains. We characterize the recombinant magnetosomes produced by these strains and demonstrate that denitrification and anaerobic photosynthesis are linked to the ability to synthesize magnetosomes upon the gene transfer. In addition, we show that the number of magnetosomes synthesized by a foreign host negatively correlates with the guanine-cytosine content difference between the host and the gene donor. Our findings have profound implications for the generation of magnetized living cells and the potential for transgenic biogenic magnetic nanoparticle production.
More Related Videos
07:37Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
Published on: May 10, 2019
09:38Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
Published on: February 2, 2021
Related Concept Videos
Export of Mitochondrial and Chloroplast Genes
Types of Genetic Transfer Between Organisms
Other Unique Bacteria
Translocation of Proteins into the Mitochondria
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Horizontal Gene Transfer
Genome Size and the Evolution of New Genes