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Identification and functional analysis of circRIPK2 in lipopolysaccharide induced chicken macrophages
1College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Abstract:
1. It was hypothesised that a circular RIPK2 (circRIPK2) highly expressed in chicken macrophages plays an important role during bacterial infection.2. After PCR amplification, Sanger sequencing and RNase R exonuclease treatment of chicken macrophages, it was found that circRIPK2 was a stable circular RNA, which was formed by reverse splicing of exons 4 to 9 of the RIPK2.3. The circRIPK2 can promote the lipopolysaccharide (LPS) induced cellular injury by reducing cell viability and increasing the expression of pro-inflammatory cytokines and apoptosis genes.4. Six miRNAs were identified as interacting with circRIPK2, potentially targeting 1,817 genes, which were significantly enriched in the Wnt signalling pathway, adherens junction and NOD-like receptor signalling pathway.5. This study provides better understanding of the function of circRIPK2, which may prove a potential biomarker and indicate potential targets for the treatment of bacterial infection.
Insights
Circular RIPK2 (circRIPK2) promotes bacterial infection-induced cellular injury in chicken macrophages by increasing inflammation and apoptosis. This circular RNA may serve as a biomarker for bacterial infections.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are increasingly recognized for their roles in gene regulation.
- Chicken macrophages are crucial in the innate immune response to bacterial pathogens.
- The specific function of circRIPK2 in chicken immunity remains largely unexplored.
Purpose of the Study:
- To investigate the role of circular RIPK2 (circRIPK2) in chicken macrophages during bacterial infection.
- To elucidate the molecular mechanisms underlying circRIPK2's function.
- To identify potential therapeutic targets for bacterial infections.
Main Methods:
- Identification and characterization of circRIPK2 using PCR, Sanger sequencing, and RNase R treatment.
- Assessment of circRIPK2's effect on cellular injury, viability, inflammation, and apoptosis.
- Bioinformatic analysis to identify miRNA interactions and enriched signaling pathways.
Main Results:
- circRIPK2 was confirmed as a stable circular RNA derived from RIPK2 exons 4-9.
- circRIPK2 exacerbated lipopolysaccharide (LPS)-induced cellular injury by reducing cell viability and increasing pro-inflammatory and apoptosis gene expression.
- Six miRNAs were found to interact with circRIPK2, targeting 1,817 genes enriched in Wnt signaling, adherens junction, and NOD-like receptor pathways.
Conclusions:
- circRIPK2 plays a significant role in promoting cellular injury during bacterial infection in chicken macrophages.
- circRIPK2 influences key immune signaling pathways through miRNA interactions.
- circRIPK2 represents a potential biomarker and therapeutic target for bacterial infections.
