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Updated: Jul 16, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Micropillar Array Based Microfluidic Device for Rare Cell Detection and Single-Cell Proteomics.

Kangfu Chen1,2, Zongjie Wang1,2

  • 1Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, Evanston, IL 60208, USA.

Methods and Protocols
|September 22, 2023
PubMed
Summary
This summary is machine-generated.

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A new microfluidic device efficiently isolates rare cells, like circulating tumor cells (CTCs), for single-cell proteomic analysis. This method enhances detection and collection, crucial for studying low-concentration samples.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Single-cell technologies enable rare cell analysis, including circulating tumor cells (CTCs) and immune cells.
  • Single-cell mass spectrometry (scMS) offers direct transcript measurement without reagents.
  • Efficient sample preparation is critical for scMS success, as protein loss impacts low-concentration samples.

Purpose of the Study:

  • To develop a microfluidics-based system for highly efficient rare cell isolation, detection, and collection.
  • To address the challenge of protein loss in single-cell proteomic analysis of rare cells.
  • To enable subsequent proteomic analysis of isolated rare cells, such as CTCs.

Main Methods:

  • Fabrication of a microfluidic device utilizing a micropillar array.
Keywords:
CTC collectionCTC isolationmicrofluidicsrare cellssingle-cell proteomics

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Last Updated: Jul 16, 2025

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  • Application of the device for the isolation of rare cells, specifically CTCs.
  • Integration of detection and collection steps for subsequent proteomic analysis.

    • Main Results:

    • Demonstration of a microfluidics-based method for efficient rare cell handling.
    • Successful isolation, identification, and collection of CTCs using the developed device.
    • Establishment of a system crucial for advancing single-cell proteomic analysis.

    Conclusions:

    • The proposed microfluidic device provides an effective solution for rare cell isolation and collection.
    • This technology is vital for improving the efficiency and success of single-cell proteomic studies.
    • The method facilitates downstream proteomic analysis of rare cells like CTCs.