Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiplexed optoacoustic tracking and magnetic actuation of labeled blood cells in living mice.

Science advances·2026
Same author

Comparative Prefrontal Multimodal Physiological Signatures Under Active- and Passive-Fatigue-Inducing Simulated Driving Paradigms.

Brain sciences·2026
Same author

Double-helix optical point spread function enables real-time mesoscopic 3D functional microangiography in the living mouse brain and skull.

Nature communications·2026
Same author

Sabotaged Integral HSC Heterogeneity Underlies Essential Thrombocythemia Development.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

A multilevel impact study of Chinese adolescents' sports participation based on ecological models of health behavior: a structural equation model analysis.

Frontiers in psychology·2025
Same author

Single-photon CASSI: towards ultralow-light spectral imaging.

Optics letters·2025
Same journal

Kat5 deficiency in alveolar type II cells licenses STAT6-driven glycolytic reprogramming and pulmonary fibrosis.

Nature communications·2026
Same journal

Continuous nonthermal slab gap formed by progressive tearing beneath Northeast Asia.

Nature communications·2026
Same journal

Zeolitic isolated protonic acid sites-mediated NH<sub>3</sub> storage for robust NO<sub>x</sub> removal.

Nature communications·2026
Same journal

Coaxially nested component with asymmetric fiber resonant cavity and separation membrane for gaseous and dissolved gases detection.

Nature communications·2026
Same journal

Near-unity charge readout signal in a nonlinear resonator without matching the sensor dissipation.

Nature communications·2026
Same journal

Prokaryotic Schlafen proteins cleave tRNAs during type III CRISPR immunity.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Jul 16, 2025

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
09:31

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

3.1K

High-resolution single-photon imaging with physics-informed deep learning.

Liheng Bian1,2, Haoze Song3, Lintao Peng3

  • 1MIIT Key Laboratory of Complex-field Intelligent Sensing, Beijing Institute of Technology, Beijing, 100081, China. bian@bit.edu.cn.

Nature Communications
|September 22, 2023
PubMed
Summary
This summary is machine-generated.

Deep learning enhances single-photon imaging using SPAD sensors, improving resolution and image quality. This method effectively reduces noise and reconstructs high-detail images from low-quality inputs.

More Related Videos

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States
06:25

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

1.0K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

Related Experiment Videos

Last Updated: Jul 16, 2025

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
09:31

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

3.1K
Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States
06:25

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

1.0K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

Area of Science:

  • Photonics and Imaging Science
  • Artificial Intelligence in Scientific Instrumentation
  • Deep Learning for Signal Processing

Background:

  • High-resolution single-photon imaging is hindered by hardware complexity and noise.
  • Existing methods struggle with low bit depth, low resolution, and significant noise in SPAD data.

Purpose of the Study:

  • To develop a deep learning framework for super-resolution single-photon imaging using SPAD sensors.
  • To enhance bit depth and overall imaging quality in challenging SPAD imaging scenarios.

Main Methods:

  • Characterized SPAD electronics noise using a complex photon flow model.
  • Created and utilized large-scale synthetic datasets for training deep learning models.
  • Developed a deep transformer network with self-attention and gated fusion for noise removal and detail extraction.

Main Results:

  • Successfully demonstrated super-resolution imaging with enhanced bit depth and quality.
  • The deep transformer network effectively removed multi-source noise and recovered full-frequency details.
  • Achieved state-of-the-art performance in experimental applications like microfluidic inspection and Fourier ptychography.

Conclusions:

  • Deep learning integration significantly advances SPAD-based super-resolution imaging capabilities.
  • The proposed method offers a robust solution for overcoming limitations in low-quality single-photon data.
  • This technique holds promise for various applications requiring high-fidelity imaging from SPAD sensors.