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Updated: Jul 16, 2025

3D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography
Published on: December 19, 2017
Ultrastructure and 3D reconstruction of a diplonemid protist (Diplonemea) and its novel membranous organelle
Daria Tashyreva1, Jiří Týč1, Aleš Horák1,2
1Institute of Parasitology, Biology Centre, Czech Academy of Sciences , České Budějovice, Czech Republic.
Importance:
The knowledge of cell biology of a eukaryotic group is essential for correct interpretation of ecological and molecular data. Although diplonemid protists are one of the most species-rich lineages of marine eukaryotes, only very fragmentary information is available about the cellular architecture of this taxonomically diverse group. Here, a large serial block-face scanning electron microscopy data set complemented with light and fluorescence microscopy allowed the first detailed three-dimensional reconstruction of a diplonemid species. We describe numerous previously unknown peculiarities of the cellular architecture and cell division characteristic for diplonemid flagellates, and illustrate the obtained results with multiple three-dimensional models, comprehensible for non-specialists in protist ultrastructure.
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