Related Experiment Video
Updated: Jul 16, 2025
07:45
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
Published on: November 28, 2015
35.6K
Polylactide Degradation Activates Immune Cells by Metabolic Reprogramming
Chima V Maduka1,2,3, Mohammed Alhaj4, Evran Ural2,3
1Comparative Medicine & Integrative Biology, Michigan State University, East Lansing, MI, 48824, USA.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 22, 2023
Summary
Polylactide (PLA) degradation causes inflammation by altering cell metabolism. Targeting this metabolic reprogramming can improve biocompatibility for medical implants.
Area of Science:
- Biomaterials Science
- Immunology
- Cellular Metabolism
Background:
- Polylactide (PLA) is a common medical biopolymer.
- PLA degradation triggers chronic inflammation and fibrosis, limiting its use.
- Current methods to neutralize acidity haven't fully resolved adverse immune responses.
Purpose of the Study:
- Investigate delayed cellular changes during PLA degradation.
- Identify mechanisms behind immune cell activation and inflammation.
- Explore metabolic reprogramming as a target for immunomodulation.
Main Methods:
- Utilized a bioenergetic model to study cellular responses to PLA degradation products.
- Analyzed amorphous and semi-crystalline PLA degradation, including l-lactic acid.
- Assessed the impact of inhibiting metabolic reprogramming and altering bioenergetics.
Main Results:
- PLA degradation products remodel cellular metabolism, creating a pro-inflammatory microenvironment.
- Elevated proinflammatory cytokines were observed due to metabolic changes.
- Inhibiting metabolic reprogramming reduced pro-inflammatory cytokines and increased anti-inflammatory signals.
Conclusions:
- Cellular metabolism is a key factor in the immune response to PLA.
- Targeting metabolic reprogramming offers a new strategy for enhancing biomaterial biocompatibility.
- This approach can improve the safety and efficacy of PLA-based medical devices.
Related Concept Videos
02:58Regulated Protein Degradation
7.4K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.4K
01:23Role of Matrix Metalloproteases in Degradation of ECM
2.4K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.4K