Related Experiment Video
Updated: Jul 16, 2025
09:27
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
17.4K
Novel Biocatalysts from Specialized Metabolism.
Hajo Kries1,2, Felix Trottmann3, Christian Hertweck3,4
1Junior Research Group Biosynthetic Design of Natural Products, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Beutenbergstr. 11a, 07745, Jena, Germany.
Angewandte Chemie (International Ed. in English)
|September 22, 2023
Summary
This study explores novel biocatalysts inspired by biosynthesis to overcome limitations in laboratory synthetic chemistry. These enzymes enable challenging reactions like amide formation and C-H activation, advancing synthetic biology.
Area of Science:
- Biocatalysis and Synthetic Chemistry
- Enzyme Discovery and Engineering
Background:
- Enzymes are valuable (bio)catalysts but their synthetic application range is limited.
- Novel biocatalyst discovery is crucial for expanding laboratory biotransformations.
Purpose of the Study:
- To provide an overview of biosynthesis-inspired discovery of novel biocatalysts.
- To highlight enzymes addressing diverse synthetic challenges.
Main Methods:
- Review of literature on biosynthesis-inspired enzyme discovery.
- Analysis of prominent enzyme examples and their catalytic functions.
Main Results:
- Discovery of novel biocatalysts for protecting-group-free amide formation and modification.
- Enzymes enabling stereoselective cyclizations, atroposelective aryl couplings, and C-H activations.
- Exploration of unusual functions in cytochrome P450s, radical SAM enzymes, and flavoproteins.
Conclusions:
- Biosynthesis-inspired enzyme discovery offers powerful solutions for synthetic challenges.
- These novel biocatalysts advance synthetic biology, enzyme engineering, and catalyst design.
- Expanding the enzyme toolbox enhances capabilities in modern organic synthesis.
More Related Videos
Related Concept Videos
01:24Biosynthesis in Bacteria
32
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
32
01:18Amino Acid Catabolism
43
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
43
01:25Lipid Catabolism
39
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
39
01:40Overview of Metabolism
30.7K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
30.7K
00:46Bioremediation
18.8K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.8K
01:28Microbial Nutrition
49
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
49