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Standardization of Food Allergen Measurements Using Multiplex Array Technology.

Kristina Reid Black1, Stephanie C Filep1, Bryan R E Smith1

  • 1InBio, Charlottesville, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2023
PubMed
Summary

Multiplex suspension array technology allows for the simultaneous measurement of multiple protein analytes. This study details a fluorescent multiplex array for quantifying specific food allergens using monoclonal antibodies and purified allergens.

Keywords:
Allergen standardizationFood allergensLuminex xMAP®MARIA® for FoodsMultiplex array

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Area of Science:

  • Food science
  • Analytical chemistry
  • Immunotechnology

Background:

  • Multiplex suspension array technology enables the simultaneous detection of multiple protein analytes within a single assay.
  • Accurate quantification of food allergens is crucial for regulatory compliance and consumer safety.

Purpose of the Study:

  • To describe a polystyrene bead-based fluorescent multiplex array for the quantitative measurement of specific food allergens.
  • To detail the standardization of array components for reliable allergen detection.

Main Methods:

  • Utilized multiplex suspension array technology with polystyrene beads and fluorescent detection.
  • Employed monoclonal antibodies for specific allergen capture and purified allergens as reference standards.
  • Developed a standardized assay for quantitative food allergen measurement.

Main Results:

  • The developed array enables the simultaneous measurement of multiple protein analytes in a single assay.
  • The array successfully quantifies specific food allergens using a standardized method.
  • The system is capable of measuring up to 17 regulated food allergens.

Conclusions:

  • Polystyrene bead-based fluorescent multiplex array technology provides a robust platform for quantitative food allergen detection.
  • Standardization of array components ensures reliable and accurate measurement of multiple food allergens.
  • This method supports regulatory requirements for food allergen testing across major global markets.