In vivo functional brain mapping using ultra-high-field fMRI in awake common marmosets

The common marmoset (Callithrix jacchus) is gaining attention in the field of cognitive neuroscience. The development of an effective protocol for fMRI data acquisition in awake marmosets is a key factor in developing reliable comparative studies. Here, we describe a protocol to obtain fMRI data in awake marmosets using auditory and visual stimulation. We describe steps for surgical and anesthesia procedures, MRI training, and positioning the marmosets within an MRI-compatible body restraint. We then detail fMRI scanning and preprocessing of functional images. For complete details on the use and execution of this protocol, please refer to Jafari et al. (2023).¹.