Pyrolyzed sediment accelerates electron transfer and regulates rhodamine B biodegradation

Electron transfer efficiency is a key factor that determined the removal of environmental pollution through biodegradation. Electron shuttles exogenously addition is one of the measures to improve the electron transfer efficiency. In this study, the sediment was pyrolyzed at different temperature to investigate its properties of mediating electron transfer and removing of rhodamine B (RhB) in microbial electrochemical systems (MESs). Sediments pyrolyzed at 300 °C (PS300) and 600 °C (PS600) have promoted electron transfer which led to 16 % enhancement of power generation while the result is reversed at 900 °C (PS900). Although power output of PS300 and PS600 are similar, the removal efficiency of RhB is not consistent, which may be caused by the biofilm structure difference. Microbial community analysis revealed that the abundance of EAB and toxicity-degrading bacteria (TDB) in PS600 was 6 % higher than that in PS300. The differentiation of microbial community also affected the metabolic pathway, the amino synthesis and tricarboxylic acid cycle were primarily upregulated with PS600 addition, which enhanced the intracellular metabolism. However, a more active cellular anabolism occurred with PS300, which may have been triggered by RhB toxicity. This study showed that pyrolytic sediment exhibits an excellent ability to mediate electron transport and promote pollutant removal at 600 °C, which provides a techno-economically feasible scenario for the utilization of low-carbon-containing solid wastes.