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Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Updated: Jul 16, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Phage engineering for development of diagnostic tools.

Subha Sankar Paul1

  • 1Syngene International Private Limited, Bangalore, India.

Progress in Molecular Biology and Translational Science
|September 22, 2023
PubMed
Summary

Phage-based diagnostics offer a cost-effective and efficient alternative to antibody-based tests. These advanced methods utilize bacteriophages for rapid pathogen detection and signal amplification, overcoming limitations of traditional assays.

Keywords:
CRISPR-Cas systemPhage typingReporter phagegenome rebootinghomologous recombinationmycobacteriophagesphage genome

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Area of Science:

  • Microbiology
  • Biotechnology
  • Diagnostics

Background:

  • Antibody-based diagnostics require cold storage and incur high costs due to production and purification.
  • Bacteriophages offer a promising alternative for pathogen detection, overcoming limitations of conventional methods.

Approach:

  • This review explores phage typing, phage-mediated detection assays, and signal amplification techniques.
  • It also discusses reporter phage engineering, genome editing platforms, and recent advancements in phage-based diagnostics.

Key Points:

  • Phage-based diagnostics leverage bacteriophages for pathogen detection, offering rapid and sensitive results.
  • Engineering reporter phages and utilizing genome editing platforms enhance detection capabilities.
  • Phage research is driving innovation in ultra-sensitive detection of various bio-species.

Conclusions:

  • Phage-based diagnostics present significant advantages over antibody-based methods, including reduced costs and simplified storage.
  • These assays are crucial for developing next-generation diagnostic tools for diverse applications.