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Updated: Jul 16, 2025

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology
Published on: May 16, 2022
Chitosan/polyacrylic acid/octadecene double-crosslinked network hydrogel functionalized porous silica microspheres
Kaixing Luo1, Yaya Gao1, Yuefei Zhang1
1School of Chemistry and Environmental Engineering, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Key Laboratory of Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan 430205, China.
Abstract:
In this study, chitosan (CS) and polyacrylic acid (PAA) were used to construct a double-crosslinked network hydrogel, which was employed as the functional material for silica microspheres to prepare a CS/PAA hydrogel modified liquid chromatographic stationary phase. During preparation, octadecene (ODE) was introduced into the CS/PAA hydrogel to improve its hydrophobicity and separation ability. The electrostatic interaction between the amino group of CS and the carboxyl group of PAA effectively prevented the swelling of the CS/PAA hydrogel, which ensured the successful application of the obtained CS/PAA hydrogel@SiO2 in chromatographic analysis. Polar nucleosides/bases and B-vitamins were selectively separated using hydrophilic interaction liquid chromatography. Hydrophobic polycyclic aromatic hydrocarbons and alkylphenols were effectively separated through reversed-phase liquid chromatography. Moreover, the effective separation of aromatic positional isomers and chiral enantiomers was achieved. This study confirms the potential application of the CS/PAA hydrogel in chromatographic separation. What is noteworthy is that the method developed in this study also provides a feasible strategy to solve the swelling issue associated with the hydrogel-based liquid chromatographic stationary phase.
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