Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

57.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.4K
RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and clustering analysis of drug-responsive temporally varying genes through high-frequency longitudinal RNA sequencing.

Scientific reports·2026
Same author

Salunke GCRI-Ahmedabad radionuclide-guided surgery: Intraoperative gamma-camera mapping for giant cell tumors around the knee.

Surgical oncology·2026
Same author

vIRA Inhibition of Antiviral Necroptosis and RIPK3 Binding Are Separable Events.

Pathogens (Basel, Switzerland)·2026
Same author

Adjuvant nivolumab and relatlimab in stage III/IV melanoma: the randomized phase 3 RELATIVITY-098 trial.

Nature medicine·2025
Same author

Novel methods for temporally varying gene identification in longitudinal studies reveal bleeding and clotting pathway activation caused by blood draws.

Briefings in bioinformatics·2025
Same author

Rare Case of Multifocal Intraosseous Cavernous Hemangioma Posing as Malignancy.

Indian journal of nuclear medicine : IJNM : the official journal of the Society of Nuclear Medicine, India·2025

Related Experiment Video

Updated: Jul 15, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.3K

Rapid, tunable, and multiplexed detection of RNA using convective array PCR.

Andrew T Sullivan1, Vibha Rao1, Tyler Rockwood1

  • 1Torus Biosystems, Inc., Medford, MA, USA.

Communications Biology
|September 23, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces convective array PCR (caPCR) for rapid, simultaneous detection of 28 RNA targets in under 40 minutes. This technology offers programmable sequence selectivity for diverse RNA detection, including viral variants.

More Related Videos

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K
Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

17.4K

Related Experiment Videos

Last Updated: Jul 15, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.3K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K
Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

17.4K

Area of Science:

  • Molecular Biology
  • Nucleic Acid Detection
  • Virology

Background:

  • Current RNA detection methods like RT-qPCR are limited in target scope, while RNA sequencing is time-consuming.
  • There is a need for rapid, high-throughput RNA detection capable of identifying specific sequences and variants.

Purpose of the Study:

  • To develop and demonstrate a novel technology for simultaneous amplification and detection of multiple RNA targets.
  • To enable programmable sequence selectivity for RNA detection, including single nucleotide variants and diverse viral strains.

Main Methods:

  • Development of convective array PCR (caPCR) technology for simultaneous amplification and detection.
  • Integration of tunable strand displacement probes for programmable sequence selectivity.
  • Application of caPCR with tunable probes for detecting multiple RNA targets from a single sample.

Main Results:

  • Simultaneous detection of 28 distinct RNA targets achieved in under 40 minutes from a single RNA sample.
  • Tunable probes demonstrated robust detection against homologous sequences and sequence diversity (e.g., mutations).
  • Proof-of-concept detection of 7 human coronaviruses and 7 SARS-CoV-2 variants of concern in a single assay.

Conclusions:

  • Convective array PCR (caPCR) with tunable probes offers a rapid and versatile platform for multiplexed RNA detection.
  • The technology enables precise identification of specific RNA sequences and detection of sequence variations, including viral mutations.
  • This approach significantly advances RNA detection capabilities, particularly for applications in virology and infectious disease surveillance.