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Optimizing cryopreservation of nasal polyp tissue for cellular functional studies and single-cell RNA sequencing
Aaqib Sohail1,2, Carolyn H Baloh1,2, Jonathan Hacker2
1Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
International Forum of Allergy & Rhinology
|September 24, 2023
Abstract
Key Points:
Mast cell numbers were reduced in samples cryopreserved as whole tissue chunks. Thawed epithelial cells had reduced proliferation rates when preserved as dissociated cell suspensions. The right cryopreservation method to choose may depend on the goals and cell-type focus of the project.
Keywords:
eosinophilic rhinitis and nasal polyposisinnate immunityparanasal sinus diseasesrhinosinusitis
