Differential Gene Expression Responses to Salt and Drought Stress in Tall Fescue (Festuca arundinacea Schreb.)

Understanding gene expression kinetics and the underlying physiological mechanisms in stress combinations is a challenge for the purpose of stress resistance breeding. The novelty of this study is correlating the physiological mechanisms with the expression of key target genes in tall fescue under a combination of various salinity and osmotic stress treatments. Four drought- and salt-responsive genes belonging to different crucial pathways evaluated included one transcription factor FabZIP69, one for the cytosolic polyamine synthetase FaADC1, one for ABA signaling FaCYP707A1, and another one for the specific Na⁺/H⁺ plasma membrane antiporter FaSOS1 involve in osmotic homeostasis. FaSOS1, FaCYP707A1, and FabZIP69 were induced early at 6 h after NaCl treatment, while FaSOS1 and FaCYP707A1 were transcribed gradually after exposure to PEG. However, stress interactions showed a significantly increased expression in all genes. Expression of these genes was positively correlated to Pro, SSs, IL, DPPH, and antioxidant enzyme activity and negatively correlated with RWC, total Chl, and MSI. Chemical analyses showed that tall fescue plants exposed to the combination of stresses exhibited increased quantity of reactive oxygen species (H₂O₂), EL and DPPH, and higher levels of antioxidant enzyme activities (CAT, and SOD), Pro, and SSs content, compared with control seedlings. Under dual-stress conditions, the expression of FabZIP69 was effective in controlling the expression of FaSOS1 and FaADC1 genes differently.