Related Experiment Video
Updated: Jul 15, 2025

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII
Published on: May 4, 2020
Multielement analysis of single red blood cells by single cell - inductively coupled plasma tandem mass spectrometry
Mathieu Melczer1, Javier Jiménez Lamana2, Ana Justo-Vega3
1French Research and Safety Institute for the Prevention of Occupational Accidents and Diseases (INRS), Toxicology and Biomonitoring Division, 1 Rue Morvan, F-54519, Vandoeuvre les Nancy, France.
Abstract:
A method for the analysis of essential metals (Fe, Cu, Mg, and Zn) and non-metals (P, S) in single red blood cells was developed by single cell (SC)-ICP-MS. The use of a triple quadrupole configuration (MS/MS) enabled an effective elimination of polyatomic interferences, which affect the accuracy of ICP-MS analysis using a single quadrupole mass analyzer. Fixation with glutaraldehyde for at least 90 days was developed to improve the quantification of elements in a single red blood cell. The experimental conditions were optimized while special attention was paid to the residence time of analytes in the plasma. Addition of a surfactant (0.05% (v/v) Tween80®) improved quantification of elements in fixed red blood cells. The detection limits obtained by SC-ICP-MS/MS were lower than for ICP-MS, especially for S and P (3 fg and 1.7 fg. cell-1 instead of 163 and 6.3 fg. cell-1, respectively).
More Related Videos
07:55Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
Published on: June 21, 2019
08:19Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
Published on: September 4, 2010
Related Concept Videos
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Atomic Emission Spectroscopy: Lab
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Atomic Emission Spectroscopy: Overview