Related Experiment Video
Updated: Jul 15, 2025
Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
Published on: January 31, 2025
Serum protein profile analysis via label-free quantitation proteomics in patients with early-onset preeclampsia
Qinghong Ji1, Shulin Zhang2, Wen Jiang3
1Department of Obstetrics, The Second Hospital of Shandong University, Jinan, Shandong, P.R. China.
Insights
This study identified key serum protein differences in early-onset preeclampsia (PE). LPA and CSH1 show potential as diagnostic and therapeutic biomarkers for this serious pregnancy complication.
Area of Science:
- Proteomics
- Biochemistry
- Maternal-Fetal Medicine
Background:
- Early-onset preeclampsia (PE) presents significant clinical challenges due to its complexity and rapid progression.
- PE poses life-threatening risks to both mother and fetus.
- Investigating serum proteome profiles is crucial for understanding early-onset PE.
Purpose of the Study:
- To identify differential serum proteome profiles in patients with early-onset PE.
- To explore potential protein biomarkers for early-onset PE.
Main Methods:
- Serum samples were analyzed using liquid chromatography and mass spectrometry.
- Bioinformatics identified functional categories and signaling pathways of differentially abundant proteins.
- Enzyme-linked immunosorbent assay (ELISA) verified key protein candidates.
Main Results:
- 30 proteins were upregulated and 34 downregulated in early-onset PE patients compared to controls.
- Differentially expressed proteins were associated with immune response and peptidase activity.
- Lower CSH1 and higher LPA concentrations were confirmed in early-onset PE patients.
Conclusions:
- This study highlights critical serum protein alterations in early-onset PE.
- LPA and CSH1 show promise as biomarkers for early-onset PE diagnosis and treatment.
Background:
Preeclampsia (PE) is a serious pregnancy complication, resulting in potentially life-threatening conditions for both mother and foetus. It is worth noting that early-onset PE has become a great challenge for clinicians due to its complex manifestation, rapid progression and serious complications. This study aims to investigate differential serum proteome profiles in patients with early-onset PE.
Methods:
Each serum sample was separated using a nanoliter flow rate Easy-nLC chromatography system. Then the samples were analysed by mass spectrometry. Bioinformatics analyses were conducted to analyse the functional categories or signal transduction pathways for differentially abundant proteins. Key proteins identified by mass spectrometry were verified by ELISA.
Results:
We found 30 and 34 proteins were upregulated and downregulated in early-onset PE patients (n = 3) vs controls (n = 3), respectively. Functional enrichment analysis revealed differentially expressed proteins related to the immune response and regulation of peptidase activity. ELISA confirmed that there were lower CSH1 levels and higher LPA concentrations in the serum samples of early-onset PE patients (n = 22) than in healthy controls (n = 19) (p < 0.05 for CSH1 and p < 0.001 for LPA).
Conclusions:
This study revealed the critical features of serum proteins in early-onset PE patients. LPA and CSH1 may serve as biomarkers for early-onset PE diagnosis and therapy.
More Related Videos
13:00Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
Published on: April 17, 2012
07:08A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
Published on: October 20, 2016