Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

15.6K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.6K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.0K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.0K
DNA-only Transposons02:57

DNA-only Transposons

14.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of the transcription factor Wor2 in biofilm formation of <i>Candidozyma auris</i>.

mSphere·2026
Same author

Manipulation of regulators of morphogenesis is not sufficient to render a <i>Candida albicans</i> colonizer strain pathogenic.

mBio·2026
Same author

Dynamic expression of candidalysin facilitates oral colonization of Candida albicans in mice.

Nature microbiology·2025
Same author

Impact of milbemycin oxime on fluconazole resistance in <i>Candida auris</i>.

JAC-antimicrobial resistance·2025
Same author

Exploration of novel mechanisms of azole resistance in <i>Candida auris</i>.

Antimicrobial agents and chemotherapy·2024
Same author

Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i>.

Microbiology spectrum·2024

Related Experiment Video

Updated: Jul 15, 2025

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

10.8K

Hijacking Transposable Elements for Saturation Mutagenesis in Fungi.

Sanne Schrevens1, Dominique Sanglard1

  • 1Institute of Microbiology, University of Lausanne and Lausanne University Hospital, Lausanne, Switzerland.

Frontiers in Fungal Biology
|September 25, 2023
PubMed
Summary

Transposable elements, or the mobilome, are key research tools for creating fungal mutant libraries. These tools accelerate the discovery of essential genes and drug targets in fungi.

Keywords:
Candidafungal pathogensfungimutagenesistransposonsyeast

More Related Videos

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
08:48

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Published on: January 26, 2017

15.5K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.0K

Related Experiment Videos

Last Updated: Jul 15, 2025

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

10.8K
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
08:48

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Published on: January 26, 2017

15.5K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.0K

Area of Science:

  • Genomics
  • Molecular Biology
  • Mycology

Background:

  • Transposable elements (mobilome) are ubiquitous in genomes and increasingly used for mutagenesis.
  • Fungi play vital roles in ecosystems, food, and human health but are understudied due to limited genetic tools.

Purpose of the Study:

  • To provide an overview of recent advancements in using transposons for saturation mutagenesis in fungi.
  • To highlight the potential of transposon insertion libraries for fungal research.

Main Methods:

  • Review of recent developments in transposon-based mutagenesis.
  • Application of transposon insertion libraries for generating extensive mutant collections.

Main Results:

  • Transposon insertion libraries offer a rapid method to study fungal genetics.
  • These libraries facilitate the identification of essential genes, compensatory mutations, and drug targets.

Conclusions:

  • Transposon technology is crucial for advancing molecular genetic studies in diverse fungal species.
  • The mobilome provides powerful tools for understanding fungal biology and developing new antifungal strategies.