Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Managing dangerous liaisons: lessons from <i>Helicobacter pylori</i> for understanding bacterial carcinogenesis.

Journal of bacteriology·2026
Same author

Metabolic cross-feeding of a dietary antioxidant enhances anaerobic energy metabolism by human gut bacteria.

Cell host & microbe·2025
Same author

An affinity-based depletion strategy for evaluating the effects of ergothioneine on bacterial physiology.

Cell chemical biology·2025
Same author

Microbial metabolism of host-derived antioxidants.

Current opinion in chemical biology·2024
Same author

Thoughts for the future.

Nature chemical biology·2024
Same author

Isotope tracing reveals bacterial catabolism of host-derived glutathione during Helicobacter pylori infection.

PLoS pathogens·2023
Same journal

Crystal Structures of Diaryl Hydrazone and Sulfone Stabilizers in Complex with an Amyloidogenic Light Chain Reveal an Alternate Ligand-Binding Cavity.

Israel journal of chemistry·2025
Same journal

Strategies for Competitive Activity-Based Protein Profiling in Small Molecule Inhibitor Discovery and Characterization.

Israel journal of chemistry·2025
Same journal

A Quantitative Sequencing Method for 5-Formylcytosine in RNA.

Israel journal of chemistry·2025
Same journal

Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation.

Israel journal of chemistry·2025
Same journal

Nanopore Direct RNA Sequencing for Modified Uridine Nucleotides Yields Signals Dependent on the Physical Properties of the Modified Base.

Israel journal of chemistry·2025
Same journal

Protein Glycosylation Patterns Shaped By the IRE1-XBP1s Arm of the Unfolded Protein Response.

Israel journal of chemistry·2025
See all related articles

Related Experiment Video

Updated: Jul 15, 2025

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
08:38

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Published on: February 22, 2019

5.9K

Activity-based Tools for Interrogating Host Biology During Infection.

Renuka Ramanathan1,2, Stavroula K Hatzios1,2,3

  • 1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 USA.

Israel Journal of Chemistry
|September 25, 2023
PubMed
Summary
This summary is machine-generated.

Activity-based protein profiling (ABPP) reveals host post-translational modifications (PTMs) that regulate cell signaling during infection. This approach identifies host targets for new therapies against pathogens.

Keywords:
Enzymeshost–microbe interactionsinfectionprotein modificationsproteomics

More Related Videos

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.9K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.3K

Related Experiment Videos

Last Updated: Jul 15, 2025

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
08:38

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Published on: February 22, 2019

5.9K
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.9K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.3K

Area of Science:

  • Cellular microbiology
  • Biochemistry
  • Immunology

Background:

  • Host cells detect and react to pathogens through dynamic cell signaling regulation.
  • Post-translational modifications (PTMs) rapidly alter protein function and interactions, modulating signaling pathways.
  • Activity-based protein profiling (ABPP) uses chemical probes to identify PTMs impacting host-pathogen interactions.

Purpose of the Study:

  • To review recent applications of ABPP in discovering host PTMs.
  • To highlight enzymes involved in host signaling modulation during infection.
  • To emphasize ABPP's role in understanding host responses and identifying therapeutic targets.

Main Methods:

  • Utilizing chemical probes for broad profiling of enzyme function.
  • Assessing side-chain reactivity to detect changes in protein activity.
  • Applying ABPP to study host PTMs in infected cells.

Main Results:

  • ABPP has been instrumental in uncovering microbial pathogenesis mechanisms.
  • Recent studies focus on ABPP's application to host-derived PTMs.
  • This technique reveals enzymes and PTMs critical for host signaling during infection.

Conclusions:

  • ABPP is a powerful tool for investigating host responses to infection.
  • The technique aids in identifying host-directed therapeutic targets.
  • Understanding host PTMs is crucial for developing novel anti-infective strategies.