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Rapid optogenetic clustering of a cytoplasmic BcLOV4 variant
Zikang Huang1, William Benman1, Liang Dong2
1Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Researchers engineered a new light-activated protein, BcLOVclust, for optogenetic control. This protein clusters in the cytoplasm faster than Cry2 and enables multiplexed control of cellular processes.
Area of Science:
- Optogenetics
- Molecular Biology
- Cell Biology
Background:
- Optogenetic protein clustering offers precise cellular control.
- Cry2 is the sole protein currently used for light-induced clustering.
- BcLOV4 induces clustering but binds to the plasma membrane, limiting its use.
Conclusions:
- BcLOVclust is a novel, versatile tool for optogenetic clustering with superior kinetics.
- It offers an alternative to Cry2 and enables multiplexed control of cellular processes.
- Further research into its thermal response is needed for application at physiological mammalian temperatures.
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