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Related Experiment Video

Updated: Jul 15, 2025

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Robust and scalable single-molecule protein sequencing with fluorosequencing.

James H Mapes¹, Julia Stover¹, Heather D Stout^1,2

¹Erisyon, Inc. Austin, TX, 78752.

Biorxiv : the Preprint Server for Biology

|September 25, 2023

Summary

This summary is machine-generated.

New fluorosequencing technology improves single molecule proteomics for clinical use. This method enhances peptide identification and quantification from limited samples, enabling sensitive neoantigen detection.

Keywords:

amino acid sequences fluorosequencing peptide labeling proteomics single-molecule protein sequencing total internal reflectance fluorescence microscopy

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Last Updated: Jul 15, 2025

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

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Published on: June 20, 2020

Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Background:

Advancements in single molecule proteomics are crucial for sensitive protein analysis, especially with limited clinical samples.
Existing methods face challenges in sensitivity and throughput for complex biological samples.
The development of novel sequencing technologies is essential to meet these demands.

Approach:

Substantial improvements to fluorosequencing, a single molecule peptide sequencing platform.
Identification of novel fluorophores compatible with sequencing chemistry.
Mitigation of dye-dye interactions using extended polyproline linkers.
Development of an integrated workflow for sample preparation and sequencing.

Key Points:

Demonstrated successful fluorosequencing of peptides in complex mixtures.
Enabled identification of a target neoantigen from a database of decoy MHC peptides.
Highlighted the platform's potential for high-sensitivity clinical applications.

Conclusions:

Fluorosequencing advancements offer a promising tool for sensitive peptide identification and quantification.
The optimized workflow and chemistry enhance the applicability of single molecule proteomics.
This technology holds significant potential for clinical diagnostics and biomarker discovery.