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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jul 15, 2025

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Photoswitchable binders enable temporal dissection of endogenous protein function.

Michael Westberg, Daesun Song, Vandon Duong

Biorxiv : the Preprint Server for Biology

|September 25, 2023

Summary

This summary is machine-generated.

Researchers developed photoswitchable designed ankyrin repeat proteins (psDARPins) for precise optical control of protein activity in living cells. This method allows researchers to study protein function with unprecedented spatiotemporal resolution.

Area of Science:

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Molecular Biology
Cell Biology
Biochemistry

Background:

Studying endogenous protein function requires precise spatiotemporal control over protein activity.
Existing methods for controlling synthetic protein binders lack generalizability.

Conclusions:

psDARPins offer a versatile and generalizable strategy for precise spatiotemporal dissection of endogenous protein function.
This technology enables new avenues for investigating cellular signaling pathways and protein interactions.