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Updated: Jul 15, 2025

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Published on: May 12, 2023
Enhancing Chromatographic Analysis: A Novel Cryo-Enrichment Module for GC Analysis of Terpenes in Cannabis sativa
Audrius Sadaunykas1, Audrius Zolumskis1, Simonas Balčiūnas1
1Department of Organic Chemistry, State Research Institute Center for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, Lithuania.
Abstract:
This study presents the development and validation of a gas chromatography (GC) method using cryo-enrichment for the analysis of α-pinene and β-caryophyllene in Cannabis sativa plant. Cryo-enrichment is a method that involves cooling part of the GC column to improve some aspect of chromatographic analysis. The aim of this study was to improve upon previous GC cryo-enrichment prototype and to create and validate a method for the analysis of these terpene compounds, which have received increasing attention for their medicinal properties. The improved cryo-enrichment device used in this study was built in-house and used a custom-built aluminum GC column holder cooled by a two-stage cryo-cooler. The validated method was found to be precise, accurate and sensitive, with good repeatability.
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