Related Experiment Video

Updated: Jul 15, 2025

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula
09:03

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula

Published on: January 26, 2024

395

Oleuropein exhibits anticarcinogen effects against gastric cancer cell lines

Mehmed Kürşad Türkdoğan1, Abdurrahim Koçyiğit2, Eray Metin Güler3

  • 1Department of Nutrition and Dietetics, Faculty of Health Sciences, Istanbul Sabahattin Zaim University, Istanbul, Turkey. kursad.turkdogan@izu.edu.tr.

Molecular Biology Reports
|September 25, 2023
PubMed
Abstract

Insights

Oleuropein (OLE), found in olives, demonstrates significant anticancer effects against gastric cancer cells in vitro. This natural compound shows potential as a future chemotherapeutic agent for gastric cancer treatment.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Oleuropein (OLE) is a key phenolic compound in olives with known health benefits.
  • Gastric cancer remains a leading cause of cancer mortality globally, often linked to dietary factors.
  • The potential of OLE as an anticancer agent against gastric cancer requires further investigation.

Purpose of the Study:

  • To investigate the cytotoxic, genotoxic, and apoptotic effects of Oleuropein (OLE) on gastric adenocarcinoma (AGS) cells in vitro.
  • To assess the role of OLE in modulating reactive oxygen species (ROS) production in gastric cancer cells.

Main Methods:

  • Gastric adenocarcinoma (AGS) cells were exposed to varying doses of OLE for 24 hours.
  • Cytotoxicity was measured using ATP viability assays.
  • Genotoxicity was assessed via Comet Assay, ROS production via H2DCF-DA assay, and apoptosis via flow cytometry.

Main Results:

  • OLE significantly reduced AGS cell viability in a dose-dependent manner, with maximum reduction around 60% at 500 µmol/L.
  • OLE induced substantial DNA damage (approx. 100%) and apoptosis with necrosis (approx. 40%) in AGS cells.
  • Increased intracellular ROS levels correlated significantly with decreased cell viability (p < 0.05–0.001).

Conclusions:

  • Oleuropein exhibits significant in vitro anticarcinogenic activity against gastric adenocarcinoma cells.
  • OLE demonstrates protective and therapeutic potential against gastric cancer.
  • Oleuropein may serve as a novel chemotherapeutic agent for future gastric cancer treatment.
Keywords:
ApoptosisDNA damageGastric cancerOleuropein

More Related Videos

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model06:12

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

Published on: May 29, 2015

7.3K
Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

753

Related Concept Videos

Cancer Prevention02:59

Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
6.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Drugs for Peptic Ulcer Disease: Sucralfate as Mucosal Protective Agents01:24

Drugs for Peptic Ulcer Disease: Sucralfate as Mucosal Protective Agents

In the intricate landscape of the gastric lumen, excessive acid secretion disrupts the natural defense mechanisms, weakening the mucus-bicarbonate barrier. This vulnerability allows pepsin to infiltrate epithelial cells, digesting mucosal proteins and triggering erosion, leading to ulcer formation.
In this scenario, mucosal protective agents like sucralfate play an essential role. Sucralfate, a complex of sulfated sucrose and aluminum hydroxide, demonstrates its usefulness in acidic conditions,...
535
Drugs for Peptic Ulcer Disease: Prostaglandin Analogs as Mucosal Protective Agents01:20

Drugs for Peptic Ulcer Disease: Prostaglandin Analogs as Mucosal Protective Agents

The gastric mucosa produces prostaglandins E2 (PGE2) and prostacyclin (PGI2), crucial in maintaining gastric health. They exert cytoprotective effects, including increasing bicarbonate secretion, releasing protective mucin, reducing gastric acid output, and preventing harmful vasoconstriction. These effects are mediated through various receptors, such as EP1, EP2, EP3, and EP4.
Non-steroidal anti-inflammatory drugs (NSAIDs) can induce peptic ulcers by inhibiting cyclooxygenase, decreasing...
478
Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists01:28

Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists

Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
503