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Accelerating Full-Thickness Wound Healing with Bacterial Cellulose-Based Multilayer Composites.
Homa Mohaghegh1, Zahra Assadi2, Amin Derakhshan2
1ACECR Institute of Higher Education (Isfahan Branch) Isfahan, P.O. Box 84175-443, Iran; Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, P.O. Box 81593-58686, Iran.
This study developed a multilayer wound dressing using bacterial cellulose, gelatin/alginate, and drug-loaded nanofibers. The innovative dressing significantly accelerated wound healing in vivo by controlling drug release and promoting tissue regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Drug Delivery Systems
Background:
- Single-layer dressings have limitations in wound healing efficacy due to restricted material properties and drug penetration.
- Multilayered approaches offer potential for simultaneous use of diverse materials and controlled drug delivery.
Purpose of the Study:
- To design and evaluate a novel multilayer wound dressing for enhanced in vivo wound healing.
- To incorporate bacterial cellulose hydrogel, gelatin/alginate hydrogel, and ciprofloxacin-loaded polycaprolactone nanofibers.
Main Methods:
- Fabrication of a multilayered wound dressing combining bacterial cellulose, gelatin/alginate, and ciprofloxacin-loaded polycaprolactone nanofibers.
- Assessment of physical properties including water absorption and water vapor transmission rate.
- In vitro drug release kinetics and antibacterial activity testing.
- In vivo evaluation of wound healing in full-thickness defects over 14 days.
Main Results:
- The multilayer dressing exhibited excellent water absorption and adequate water vapor transmission rate.
- Controlled release of ciprofloxacin was observed, providing sustained antibacterial activity against various strains.
- Fibroblast cells maintained normal morphology and metabolic activity.
- In vivo studies showed accelerated full-thickness wound healing, reduced inflammation, and enhanced skin regeneration.
Conclusions:
- The developed multilayer wound dressing effectively promotes wound healing through controlled drug release and synergistic material properties.
- This innovative approach offers a promising strategy for advanced wound management and tissue repair.
