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Thialysine utilization by thialysine resistant CHO cells.

A Di Girolamo, M Di Girolamo, C Cini

    Physiological Chemistry and Physics and Medical NMR
    |January 1, 1986
    PubMed
    Summary

    Thialysine-resistant Chinese hamster ovary (CHO) cells show reduced uptake of the amino acid analog thialysine compared to parental cells. This resistance impacts protein synthesis, with variant cells incorporating significantly less thialysine in place of lysine.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Genetics

    Background:

    • Thialysine is an analog of lysine, an essential amino acid.
    • Thialysine can be incorporated into proteins during synthesis, replacing lysine.
    • Resistance to thialysine in cell lines suggests altered amino acid metabolism or transport.

    Purpose of the Study:

    • To investigate the mechanism of thialysine resistance in Chinese hamster ovary (CHO) cells.
    • To quantify the differential utilization of thialysine between parental and resistant CHO cell lines.
    • To understand the impact of thialysine resistance on protein synthesis.

    Main Methods:

    • Cell culture of parental and thialysine-resistant CHO cell lines.
    • Quantification of thialysine uptake from culture medium.

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  • Analysis of thialysine incorporation into cellular proteins.

    • Main Results:

    • Thialysine-resistant CHO cells exhibit significantly lower utilization of exogenous thialysine compared to parental cells.
    • Parental CHO cells can substitute up to 11% of protein lysine with thialysine.
    • Variant thialysine-resistant cells incorporate a maximum of 5% thialysine in place of lysine.

    Conclusions:

    • Thialysine resistance in CHO cells is associated with reduced cellular uptake and incorporation of thialysine.
    • The observed resistance impacts the extent to which thialysine can replace lysine in protein synthesis.
    • These findings provide insights into amino acid analog resistance mechanisms in mammalian cells.