Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoperiod-dependent protein and lipid biosynthesis in Auxenochlorella pyrenoidosa cultivated using food waste hydrolysate.

Journal of environmental management·2026
Same author

Integrative Omics Analysis Reveals the Regulation of Hypoxia Tolerance in Large Yellow Croaker (Larimichthys crocea) via the Lipoic Acid Synthase (lias) Gene.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Early Predictive Value of Peripheral Inflammatory Index Combined with High-Sensitivity Troponin T for Sepsis-Induced Cardiomyopathy.

Mediterranean journal of hematology and infectious diseases·2026
Same author

High proportion of peripheral blood and haploidentical stem cell transplantation in Chinese children: a nationwide CCBMTR analysis.

Bone marrow transplantation·2026
Same author

Exosome-based strategies to enhance CAR-T cell therapy for systemic lupus erythematosus: A promising new frontier.

International reviews of immunology·2026
Same author

Donor age and sex program MSC secretome signaling: a quadrant framework for precision therapy.

Stem cell research & therapy·2026

Related Experiment Video

Updated: Jul 15, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.8K

Application of Single Extracellular Vesicle Analysis Techniques.

Junquan Zhu1, Feifeng Wu1, Cuifang Li1

  • 1Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, People's Republic of China.

International Journal of Nanomedicine
|September 26, 2023
PubMed
Summary

Single extracellular vesicle (SEV) analysis overcomes limitations of bulk methods for studying cell-derived EVs. This review highlights SEV detection advances and their clinical applications in understanding intercellular communication and disease.

Keywords:
detection methodsdisease diagnosisheterogeneitynew therapiessingle extracellular vesicle analysis

More Related Videos

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

9.9K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.5K

Related Experiment Videos

Last Updated: Jul 15, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.8K
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

9.9K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.5K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Nanotechnology

Background:

  • Extracellular vesicles (EVs) are crucial for intercellular communication, carrying proteins and genomes from parent cells.
  • EVs play roles in physiological and pathological processes, offering diagnostic and therapeutic potential.
  • Heterogeneity in EVs complicates functional understanding, necessitating advanced analytical approaches.

Purpose of the Study:

  • To review recent advancements in single extracellular vesicle (SEV) detection methods.
  • To summarize the clinical applications of SEV detection strategies.
  • To emphasize the importance of SEV analysis for understanding EV heterogeneity and function.

Main Methods:

  • Review of current literature on SEV detection techniques.
  • Analysis of methods enabling physical and molecular characterization at the individual EV level.
  • Compilation of clinical applications stemming from SEV detection strategies.

Main Results:

  • SEV analysis techniques provide detailed insights into EV composition and physical properties, overcoming bulk analysis limitations.
  • These methods preserve information on EV heterogeneity, crucial for understanding their biological roles.
  • Various clinical applications are emerging, leveraging SEV detection for disease diagnosis and monitoring.

Conclusions:

  • Single extracellular vesicle (SEV) analysis is essential for deciphering EV heterogeneity and function.
  • Advances in SEV detection methods are expanding their utility in biomedical research.
  • SEV detection strategies show significant promise for future clinical applications in diagnostics and therapeutics.