Related Experiment Video

Updated: Jul 15, 2025

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

1.5K

E2F3/CDCA2 reduces radiosensitivity in gastric adenocarcinoma by activating PI3K/AKT pathway

Jun Gao1, Huaqiao Wang1, Xiujuan Qiu2

  • 1Department of General Surgery, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang, China.

The British Journal of Radiology
|September 26, 2023
PubMed
Abstract

Insights

The E2F3/CDCA2 axis promotes gastric adenocarcinoma progression and reduces radiosensitivity by activating the PI3K/AKT pathway. This axis represents a potential new therapeutic target for this increasing global cancer.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • Gastric adenocarcinoma is a leading cause of cancer deaths with rising incidence.
  • CDCA2, a nuclear protein, is implicated in promoting tumor growth.

Purpose of the Study:

  • To investigate the role of CDCA2 in gastric adenocarcinoma progression and radiosensitivity.
  • To elucidate the underlying molecular mechanisms involving CDCA2.

Main Methods:

  • Bioinformatic analysis to identify differentially expressed genes and upstream regulators.
  • Quantitative real-time PCR (qRT-PCR) and molecular experiments to validate regulatory relationships.
  • Cell viability (CCK-8), proliferation, and radiosensitivity assays (colony formation), and Western blot analysis of the PI3K/AKT pathway.

Main Results:

  • CDCA2 was significantly upregulated in gastric adenocarcinoma, promoting proliferation and decreasing radiosensitivity.
  • The PI3K/AKT pathway inhibitor reversed the effects of CDCA2 on proliferation and radiosensitivity.
  • E2F3 was identified as an upstream transcription factor that activates CDCA2, driving proliferation and reducing radiosensitivity via the PI3K/AKT pathway.

Conclusions:

  • The E2F3/CDCA2 axis promotes gastric adenocarcinoma cell proliferation and reduces radiosensitivity.
  • This axis operates through the activation of the PI3K/AKT pathway.
  • The E2F3/CDCA2 axis presents a novel therapeutic target for gastric adenocarcinoma.

More Related Videos

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells08:54

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

14.1K
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.6K

Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.6K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
14.3K