Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

2.6K
Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
2.6K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

449
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
449
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

836
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
836
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

253
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
253
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.1K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.1K
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

2.4K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Nitroxide-Substituted Hydrazone Switch: Experimental and Theoretical Insights into Photoswitching Behavior.

ACS organic & inorganic Au·2026
Same author

Mn(III)-porphyrins in battle against neurodegenerative and cancer diseases.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Field-domain rapid-scan EPR at 240GHz for studies of protein functional dynamics at room temperature.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2024
Same author

Photoswitchable hydrazones with pyridine-based rotors and halogen substituents.

RSC advances·2024
Same author

Levamisole Based Co(II) Single-Ion Magnet.

Chemistry, an Asian journal·2024
Same author

Partial Thermal Condensation Mediated Synthesis of High-Density Nickel Single Atom Sites on Carbon Nitride for Selective Photooxidation of Methane into Methanol.

Small (Weinheim an der Bergstrasse, Germany)·2023

Related Experiment Video

Updated: Jul 15, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

10.7K

MEPROS - Modular electron paramagnetic resonance operating software for multifunctional high-frequency EPR

Matúš Šedivý1, Vinicius Santana2, Antonín Sojka2

  • 1Magneto-Optical and THz Spectroscopy, Central European Institute of Technology (CEITEC), Brno University of Technology (BUT), Czech Republic; Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Czech Republic.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|September 26, 2023
PubMed
Summary

A new LabVIEW software controls a home-built High-Frequency Electron Paramagnetic Resonance (HF-EPR) spectrometer. This versatile solution enables advanced EPR measurements like CW-EPR, FS-EPR, and 2D-EPR mapping for materials research.

Keywords:
AutomationElectron paramagnetic resonanceInstrumentationLabVIEWSoftwareZeeman diagram

More Related Videos

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.4K
In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

6.5K

Related Experiment Videos

Last Updated: Jul 15, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

10.7K
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.4K
In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

6.5K

Area of Science:

  • Spectroscopy
  • Materials Science
  • Software Engineering

Background:

  • Home-built spectrometers require tailored control software.
  • High-Frequency Electron Paramagnetic Resonance (HF-EPR) offers unique insights into material properties.
  • Developing adaptable software is crucial for evolving experimental needs.

Purpose of the Study:

  • To present a modular LabVIEW software solution for controlling a home-built HF-EPR spectrometer.
  • To implement versatile measuring procedures including Continuous Wave EPR (CW-EPR), Frequency-Swept EPR (FS-EPR), and 2D-EPR mapping.
  • To demonstrate the software's automation capabilities and adaptability for various experimental setups.

Main Methods:

  • Development of a modular software architecture in LabVIEW.
  • Implementation of control protocols for spectrometer subsystems.
  • Integration of measurement procedures for CW-EPR, FS-EPR, and 2D-EPR mapping.

Main Results:

  • Successful automation of CW-EPR, FS-EPR, and 2D-EPR measurements.
  • Acquisition of EPR spectra for Silicon Carbide doped with vanadium (SiC + V) under varying conditions.
  • Obtained dense FS-EPR data for lithium phthalocyanine crystal and multi-frequency spectra via 2D-EPR mapping.

Conclusions:

  • The developed LabVIEW software provides a flexible and automated solution for HF-EPR spectroscopy.
  • The modular design facilitates easy adaptation to hardware modifications and reuse in other experimental setups.
  • The software is actively used in research, contributing to published scientific data.