Continuous monitoring of multiple biomarkers with an ultrasensitive 3D-structured wearable biosensor

Chronic diseases call for routine management of frequent monitoring of specific biomarkers. Traditional in vitro diagnostics technologies suffer from complex sampling processes and long detection intervals, which cannot meet the need of continuous monitoring. Wearable devices taking advantage of compact size, rapid detection process, and small sample consumption are promising to take the place of endpoint detection, providing more comprehensive information about human health. Here, we proposed a fully integrated wearable system with an ultrasensitive 3D-structured biosensor for real-time monitoring of multiple metabolites. The 3D-structured biosensor shows wide linear ranges of 400-1,400 μM and 0.1-8 mM and high sensitivities of 460.5 and 283.09 μA/(mM·cm²) for lactate and glucose detection, respectively. We have conducted in vivo animal experiments, and the proposed wearable biosensor demonstrated high consistency with established methods. We envision that this system could provide a real-time wearable detection platform for multiple biomarker detection.