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Znf598-mediated Rps10/eS10 ubiquitination contributes to the ribosome ubiquitination dynamics during zebrafish
Nozomi Ugajin1, Koshi Imami2,3, Hiraku Takada1
1Department of Frontier Life Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kita-ku, Kyoto 603-8555, Japan.
RNA (New York, N.Y.)
|September 26, 2023
Summary
Ribosome ubiquitination dynamically changes during zebrafish development, regulated by Znf598 and impacting ribosomal protein Rps10/eS10. This reveals complex regulation of protein synthesis during animal growth.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- Ribosomes are essential for protein synthesis and are subject to ubiquitination, crucial for translational regulation and ribosome-associated quality control (RQC).
- The dynamics of ribosome ubiquitination during complex multicellular organism development remain largely unexplored.
Purpose of the Study:
- To investigate the dynamic changes in ribosome ubiquitination throughout zebrafish development.
- To identify the E3 ubiquitin ligase responsible for ribosome ubiquitination during development and key ubiquitinated ribosomal proteins.
Main Methods:
- Generated a zebrafish strain expressing FLAG-tagged ribosomal protein Rpl36/eL36 from its endogenous locus.
- Utilized affinity purification of ribosomes from zebrafish embryos and immunoblotting analysis to examine ribosome ubiquitination.
- Investigated the role of specific ubiquitination sites on ribosomal protein Rps10/eS10.
Main Results:
- Ribosome ubiquitination patterns exhibited dynamic changes during zebrafish development.
- The E3 ubiquitin ligase Znf598 was identified as a key regulator of ribosome ubiquitination during zebrafish development.
- Ribosomal protein Rps10/eS10 was identified as a major ubiquitinated protein, and its ubiquitination-site mutations affected overall ribosome ubiquitination.
Conclusions:
- Ribosome ubiquitination is a dynamic and complex process during animal development.
- Znf598-mediated ubiquitination of Rps10/eS10 plays a significant role in regulating ribosome function during zebrafish development.
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