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Updated: Jul 15, 2025

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting FACS
Published on: January 14, 2013
[Cancer-Associated Fibroblasts: Heterogeneity and Bimodality in Oncogenesis]
N A Lunina1,2, D R Safina1, S V Kostrov1
1National Research Center "Kurchatov Institute", Moscow, 123182 Russia.
Abstract:
Cancer-associated fibroblasts (CAFs) often form a major component of the tumor microenvironment (TMA), providing conditions for cancer cells to thrive. CAFs may contribute to tumor growth, invasion, metastasis, and resistance to therapy. However, clinical trials of treatment strategies targeting CAFs have largely failed. Moreover, there is evidence that CAFs are capable of inhibiting tumor development. The review considers the current data on the functional heterogeneity of CAFs and their bimodality in tumor development and progression. Understanding the tumor-promoting and tumor-inhibiting activities of CAFs can help to develop new diagnostic and therapeutic approaches.
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