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[DNA Methylation Profile of CD14+ Monocytes Changes in Primary Progressive Multiple Sclerosis]
I S Kiselev1,2, O G Kulakova1, O A Baturina3
1Pirogov Russian National Research Medical University, Moscow, 117997 Russia.
Molekuliarnaia Biologiia
|September 27, 2023
Summary
Primary progressive multiple sclerosis (PPMS) shows distinct DNA methylation patterns in monocytes. Most differentially methylated positions were hypermethylated, suggesting epigenetic dysregulation in this MS subtype.
Area of Science:
- Neuroimmunology
- Epigenetics
- Genomics
Context:
- Multiple sclerosis (MS) is a heterogeneous neurodegenerative disease.
- Primary progressive MS (PPMS) presents unique challenges due to steady neurodegeneration.
- Epigenetic regulation, specifically DNA methylation, is under-explored in PPMS pathogenesis.
Purpose:
- To identify differential DNA methylation profiles in CD14+ monocytes of PPMS patients.
- To investigate the role of epigenetic modifications in PPMS compared to healthy individuals.
Summary:
- Genome-wide analysis revealed 169 differentially methylated positions (DMPs) in PPMS patients' monocytes.
- Over 90% of these DMPs were hypermethylated in PPMS.
- Six differentially methylated regions (DMRs) were identified in genes linked to inflammation and neurodegeneration (OR2L13, CAT, LCLAT1, HOXA5, RNF39, CRTAC1).
Impact:
- Highlights the functional significance of epigenetic alterations in PPMS.
- Suggests active epigenetic regulation of genes involved in inflammation and neurodegeneration in PPMS.
- Provides a foundation for understanding PPMS pathogenesis and developing targeted epigenetic therapies.
