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Related Experiment Video

Updated: Jul 15, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Enzyme-Assisted Amplification and Copper Nanocluster Fluorescence Signal-Based Method for miRNA-122 Detection.

Yang Qing1, Haobin Fang1, Yuxing Yang1

  • 1State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, China.

Biosensors
|September 27, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a new miRNA detection platform using DNA and copper nanoclusters for sensitive detection of miRNA-122. The fluorescence reduction method offers high selectivity for early disease diagnosis.

Keywords:
CuNCsexonuclease IIIfluorescence reductionmiRNA-122

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Nanotechnology

Background:

  • MicroRNAs (miRNAs) are crucial biomarkers for diseases like cancer.
  • Existing miRNA detection platforms require improvement in sensitivity and selectivity.
  • Development of novel biosensors is essential for early disease diagnosis.

Purpose of the Study:

  • To develop a novel, sensitive, and selective fluorescence-based biosensor for miRNA detection.
  • To utilize miRNA-122 as a target for demonstrating the platform's efficacy.
  • To establish a circular amplification strategy for enhanced detection.

Main Methods:

  • Design of an unlabelled DNA probe (DNA1) with a T-long strand for copper nanocluster (CuNC) formation.
  • Utilizing nucleic acid exonuclease III (Exo III) for target-mediated hydrolysis and circular amplification.
  • Employing streptavidin-magnetic beads (SIBs) for separation and fluorescence signal reduction measurement.
  • Quantifying miRNA-122 by measuring the decrease in fluorescence intensity.

Main Results:

  • The biosensor demonstrated high sensitivity with a detection limit as low as 0.46 nM for miRNA-122.
  • The method exhibited excellent discrimination and selectivity for the target miRNA.
  • Fluorescence signal reduction directly correlated with the amount of hydrolyzed DNA1 and thus target miRNA-122 concentration.

Conclusions:

  • The developed platform offers a sensitive and selective approach for miRNA detection.
  • This fluorescence reduction-based method serves as a valuable tool for early disease diagnosis.
  • The technique provides a promising reference for the development of advanced miRNA biosensors.