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Microfluidic Chip Fabrication and Method to Detect Influenza
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Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection.

Andrew Murray1, Julio Ojeda1, Omar El Merhebi1

  • 1Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA.

Biosensors
|September 27, 2023
PubMed
Summary
This summary is machine-generated.

A novel electrochemical biosensor detects SARS-CoV-2 and Influenza A virus using a reusable five-stranded four-way junction (5S-4WJ) DNA system. This cost-effective sensor offers sensitive and selective pathogen detection for future infectious disease surveillance.

Keywords:
Influenza ASARS-CoV-2biosensorfour-way junctionmicroliter detection

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Area of Science:

  • Nanotechnology and Biosensing
  • Molecular Diagnostics
  • Virology

Background:

  • Accurate and rapid detection of viral pathogens like SARS-CoV-2 and Influenza A is crucial for public health.
  • Existing diagnostic methods can be time-consuming, expensive, or lack multiplexing capabilities.
  • Electrochemical biosensors offer a promising platform for sensitive and cost-effective molecular detection.

Purpose of the Study:

  • To develop a modular, reusable, and cost-effective electrochemical biosensor for detecting specific viral nucleic acid sequences.
  • To enable simultaneous detection of SARS-CoV-2 (genes S and N) and Influenza A virus (gene M) using a single platform.
  • To demonstrate the biosensor's potential for multiplexed detection of current and future pathogens.

Main Methods:

  • Fabrication of a five-stranded four-way junction (5S-4WJ) DNA structure immobilized on an electrode.
  • Utilized a universal stem-loop (USL) strand, two auxiliary DNA strands (m and f) for target specificity, and a methylene blue redox strand (UMeB).
  • Employed square wave voltammetry (SWV) for sequence-specific detection and assessed electrode reusability through a simple rinsing and re-hybridization procedure.

Main Results:

  • The 5S-4WJ biosensor demonstrated selective detection of target viral genes (SARS-CoV-2 S/N, Influenza A M).
  • The biosensor maintained high signal integrity after multiple reuse cycles, indicating excellent electrode stability.
  • Achieved a low limit of quantification (LOQ) as low as 17 pM, highlighting high sensitivity.

Conclusions:

  • The developed 5S-4WJ electrochemical biosensor is a versatile, cost-effective, and reusable platform for multiplex viral pathogen detection.
  • Its modular design and reusability make it suitable for adaptable diagnostic assays targeting various pathogens.
  • This technology holds significant potential for rapid, sensitive, and sustainable infectious disease surveillance.