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Cellular localization of the hybrid pyruvate/2-oxoglutarate dehydrogenase complex in the actinobacterium

Lea Sundermeyer¹, Jan-Gerrit Folkerts¹, Benita Lückel¹

¹IBG-1: Biotechnology, Institute of Bio- and Geosciences, Forschungszentrum Jülich , Jülich, Germany.

Microbiology Spectrum

|September 27, 2023

Abstract

Importance:

Enzymes involved in the central metabolism of bacteria are usually considered to be distributed within the entire cytoplasm. Here, we provide an example for a spatially defined localization of a unique enzyme complex of actinobacteria, the hybrid pyruvate dehydrogenase/2-oxoglutarate dehydrogenase (PDH-ODH) complex composed of four different subunits. Using fusions with mVenus or mCherry and fluorescence microscopy, we show that all four subunits are co-localized in distinct spots at the cell poles, and in larger cells, additional spots were observed at mid-cell. These results clearly support the presence of the hybrid PDH-ODH complex and suggest a similar localization in other actinobacteria. The observation of a defined spatial localization of an enzyme complex catalyzing two key reactions of central metabolism poses questions regarding possible consequences for the availability of substrates and products within the cell and other bacterial enzyme complexes showing similar behavior.

Keywords:

2-oxoglutarate dehydrogenase complex Corynebacterium glutamicum ODH inhibitory protein OdhI cellular localization fluorescence microscopy pyruvate dehydrogenase complex

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