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Long Non-Coding RNAs as "MYC Facilitators".

Daniel García-Caballero1, Jonathan R Hart1, Peter K Vogt1

  • 1Department of Molecular Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Pathophysiology : the Official Journal of the International Society for Pathophysiology
|September 27, 2023
PubMed
Summary
This summary is machine-generated.

Researchers identified "MYC facilitators," a class of long non-coding RNAs (lncRNAs) that support MYC-driven cell proliferation without being essential for cell survival. These MYC-interacting lncRNAs are key to understanding MYC

Keywords:
CRISPRiCRISPR–Cas9lncRNAstranscription factor–RNA complextranscriptional regulation

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Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • The MYC oncogene plays a critical role in cell proliferation, survival, and metabolism.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized as key regulators of gene expression and cellular processes.
  • The precise mechanisms by which MYC influences cellular functions through lncRNAs are not fully understood.

Purpose of the Study:

  • To define and characterize a specific class of lncRNAs that are directly regulated by MYC.
  • To investigate the functional role of these MYC-interacting lncRNAs in MYC-driven cellular processes.
  • To propose a hypothesis regarding the involvement of lncRNAs in MYC-mediated transcriptional regulation.

Main Methods:

  • Identification of lncRNAs based on criteria: direct transcriptional targets of MYC, coordinated expression with MYC, requirement for sustained MYC-driven proliferation, and non-essentiality for cell survival.
  • Detailed analysis of two representative lncRNAs: SNHG17 (small nuclear RNA host gene 17) and LNROP (long non-coding regulator of POU2F2).
  • Formulation of a general hypothesis on the role of lncRNAs in MYC's transcriptional regulatory network.

Main Results:

  • A distinct class of lncRNAs, termed 'MYC facilitators,' was identified.
  • These MYC facilitators are direct transcriptional targets of MYC and their expression is coordinated with MYC levels.
  • SNHG17 and LNROP were presented as key examples of MYC facilitators, crucial for sustained proliferation but not survival.

Conclusions:

  • MYC facilitates play a critical role in sustaining MYC-driven cell proliferation.
  • These lncRNAs represent a novel layer of regulation in MYC-mediated cellular processes.
  • Further research into MYC facilitators could reveal new therapeutic targets in MYC-driven cancers.