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p97/VCP Promotes the Recycling of Endocytic Cargo
Mona Kawan1, Maria Körner1, Andreas Schlosser2
1Chair of Biochemistry I, University of Würzburg, Biocenter, Am Hubland, 97074 Würzburg, Germany.
Molecular Biology of the Cell
|September 27, 2023
Summary
The ATPase p97 (VCP, Cdc48) interacts with myoferlin (MYOF) to regulate endocytic trafficking. This interaction is crucial for recycling endocytic cargo, expanding p97
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- The endocytic pathway is vital for cellular functions including material uptake, protein quality control, and signaling.
- The ATPase p97 (VCP, Cdc48) is implicated in endolysosomal degradation, but its role in endocytic trafficking remains unclear.
Purpose of the Study:
- To identify novel interactors of p97 involved in endocytic trafficking.
- To elucidate the function of myoferlin (MYOF) in the context of p97-mediated endosomal pathways.
Main Methods:
- Co-immunoprecipitation to identify protein interactions.
- Immunofluorescence microscopy to determine protein localization.
- Pharmacological inhibition assays to assess functional impact.
Main Results:
- Myoferlin (MYOF) was identified as a novel interactor of p97, dependent on the cofactor PLAA.
- p97 and PLAA were found to co-localize with MYOF, Rab11, Rab14, and CD71 in endosomal compartments.
- Inhibition of p97 function impaired transferrin recycling.
Conclusions:
- p97, through its interaction with MYOF and PLAA, plays a significant role in endosomal recycling.
- p97 is involved in both the degradation and recycling arms of the endocytic pathway.
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